Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40738-018-0046-4/MediaObjects/40738_2018_46_Fig3_HTML.gif)
Gating strategy based on pan lymphocyte marker CD45+, with positive staining for markers CD3 and CD8 and negative gating to acquire the CD4+ T-lymphocytes. Intracellular cytokine expression is then measured in the CD4+ cells. This strategy is required as CD4+ surface marker expression is suppressed by the stimulatory process