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Table 3 Classification of the phases of oestrous cycle based on the cell types and relative number of these cells in vaginal smears (Cora et al., [8])

From: Staging of the estrous cycle and induction of estrus in experimental rodents: an update

Oestrous phase

Neutrophils

Small nucleated epithelial cells

Large nucleated epithelial cells

Anucleated keratinized epithelial cells

Relative cell density

Proestrus

0 to +

++ to +++

0 to +

0 to +

Low to moderate

Estrus

 Rat

0 to +

0 to ++

0 to ++

++ to +++

Moderate to high

 Mouse

0 to +

0 to +

0 to +

++ to +++

Moderate to high

Metestrus

 Rat

+ to +++

+ to ++

+ to ++

+ to +++

Moderate to high

 Mouse

+ to +++

0 to +

0 to +

++ to +++

Moderate to high

Diestrus

++ to +++

+ to ++

+ to ++

0 to +

Low to moderate

  1. 0 = none; + = few; ++ = moderate; +++ = high