From: Staging of the estrous cycle and induction of estrus in experimental rodents: an update
Method | Procedure | Reference(s) |
---|---|---|
Common method | Subcutaneous Oestradiol benzoate 10 μg/100 g body weight 48 h prior to pairing + subcutaneous progesterone 0.5 mg/100 g body weight 4–6 h prior to pairing | |
Alternative method | Oral ethinyl oestradiol 100 μg/animal 48 h prior to pairing + subcutaneous progesterone 1 mg/animal 6 h prior to pairing | (Singh et al., [23]) |