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Table 5 Induction of estrus phase (heat)

From: Staging of the estrous cycle and induction of estrus in experimental rodents: an update

MethodProcedureReference(s)
Common methodSubcutaneous Oestradiol benzoate 10 μg/100 g body weight 48 h prior to pairing + subcutaneous progesterone 0.5 mg/100 g body weight 4–6 h prior to pairing(Amin et al., [2]; Sahoo et al., [22]; Tang et al., [26])
Alternative methodOral ethinyl oestradiol 100 μg/animal 48 h prior to pairing + subcutaneous progesterone 1 mg/animal 6 h prior to pairing(Singh et al., [23])