Fig. 2
From: MAML1: a coregulator that alters endometrial epithelial cell adhesive capacity
Examination of the effect of MAML1 knockdown on Ishikawa cell adhesive capacity. Ishikawa cells were transfected with either MAML1 siRNA (20 nM) or scrambled control (20 nM) before HTR8/SVneo spheroid adhesion assay or other analysis. a MAML1 knockdown was confirmed by qPCR. Expression levels were normalized to 18S (n = 9). b MAML1 knockdown was confirmed by immunoblotting and densitometry, normalized against a loading control GAPDH (n = 4). c MAML1 knockdown significantly compromised the spheroid adhesion compared to scrambled control (n = 5). d Representative images are presented to show attached spheroids on the Ishikawa cell monolayer after adhesion assay. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001